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To produce a stock solution of density 1.15 g/ml dilute 27.6 ml of OptiPrep to 60 ml with the chosen diluent (see Section 2c)

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The integrity of the RNA was evaluated by agarose gel electrophoresis (1.5% agarose), and the quantity and quality of RNA were verified by OD absorption ratio at 260 / 280 using Bio Spec-nano (serial no., A116449

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Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice